Form 11-K (Mark one) OR December31, 2025 and 2024 Signatures Exhibit: Exhibit 23.1 –Consent of Independent Registered Public Accounting Firm Note:Other supplemental schedules required by Section 2520.103-10 of the Department of Labor's Rules and Regulations for Reporting andDisclosure under the Employee Retirement Income Security Act of 1974 have been omitted because they are not applicable to the NovoNordisk Inc. 401(k) Savings Plan. To the Plan Administrator, Participants and Beneficiariesof the Novo Nordisk Inc. 401(k) Savings Plan Opinion on the Financial Statements We have audited the accompanying statements of net assets available for benefits of the Novo Nordisk Inc. 401(k) Savings Plan (the “Plan”) as of December31, 2025 and 2024, and the related statements of changes in net assets available for benefits forthe years then ended, and the related notes (collectivelyreferred to as the “financial statements”). In our opinion, the financial statements present fairly, in all material respects, the net assets available for benefits of the Plan as of December 31, 2025 and2024, and the changes in net assets available for benefits for each of the years then ended, in conformity with accounting principles generally accepted in theUnited States of America. Basis for Opinion These financial statements are the responsibility of the Plan's management. Our responsibility is to express an opinion on the Plan’s financial statements basedon our audits. We are a public accounting firm registered with the Public Company Accounting Oversight Board (United States) (“PCAOB”) and are requiredto be independent with respect to the Plan in accordance with the U.S. federal securities laws and the applicable rules and regulations of the Securities andExchange Commission and the PCAOB. We conducted our audits in accordance with the standards of the PCAOB. Those standards require that we plan and perform the audit to obtain reasonableassurance about whether the financial statements are free of material misstatement, whether due to error or fraud. The Plan is not required to have, nor werewe engaged to perform, an audit of its internal control over financial reporting. As part of our audits, we are required to obtain an understanding of internalcontrol over financial reporting but not for the purpose of expressing an opinion on the effectiveness of the Plan's internal control over financial reporting.Accordingly, we express no such opinion. Our audits included performing procedures to assess the risks of material misstatement of the financial statements, whether due to error or fraud, andperforming procedures that respond to those risks. Such procedures included examining, on a test basis, evidence regarding the amounts and disclosures in thefinancial statements. Our audits also included evaluating the accounting principles used and significant estimates made by management, as well as evaluatingthe overall presentation of the financial statements. We believe that our audits provide a reasonable basis for our opinion. Supplemental Information The supplemental information in the accompanying schedule of assets (held at end of year) as of December 31, 2025, has been subjected to audit proceduresperformed in conjunction with the audit of the Plan's financial statements. The supplemental information is the responsibility of the Plan's management. Ouraudit procedures included determining whether the supplemental information reconciles to the financial statements or the underlying accounting and otherrecords, as applicable, and performing procedures to test the completeness and accuracy of the information presented in the supplemental information. Informing our opinion on the supplemental information, we evaluated whether the supplemental information, including its form and content, is presented inconformity with the Department of Labor's Rules and Regulations for Reporting and Disclosure under the Employee Retirement Income Security Act of 1974.In our opinion, the supplemental information is fairly stated, in all material respects, in relation to the financial statements as a whole. /s/ EisnerAmper LLP We have served as the Plan’s auditor since 2007. EISNERAMPER LLPMetairie, LouisianaJune 17, 2026 NOVO NORDISK INC. 401(k) SAVINGS PLANNotes to Financial StatementsDecember 31, 2025 and 2024 Note1 -Description of PlanThe following description of the Novo Nordisk Inc. 401(k) Savings Plan (the “Plan”) provides only general information. Participants should refer to the Plan document, as amended, for a more complete description of the Plan's provisions. GeneralThe Plan is a participant-directed defined contribution plan which was established on January 1, 1983 and was most recently amended and restated effective as of January 1, 2024, and subsequently on January 1, 2025. Companies participating in the Plan include Catalent Indiana, LLC(“CILLC”), Dicerna Pharmaceuticals, Inc. (“NNDRNA”), Novo Nordisk Inc. (“NNI”), Novo N