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ECDCGUIDANCEPublic health guidance for assessing andmitigating the risk of locally-acquiredAedes-borne viral diseases in the EU/EEA iiThis report of the European Centre for Disease Prevention and Control (ECDC) was written and coordinated byTamás Bakonyi, Principal Expert Vector-Borne and Zoonotic Diseases, Food-, Water-, Vector-borne and ZoonoticDiseases Section, One-Health-related Diseases Unit, ECDC.Contributing authors (in alphabetical order)External experts: Luisa Barzon (Department of Molecular Medicine, University of Padova, Italy), Clémentine Calba(Santé publique France, Marseille, France), Bruna Raquel Figueira Ornelas de Gouveia (Regional Health Directorate,Autonomous Region of Madeira, Funchal, Portugal), Antonios Michaelakis (Scientific Directorate of Entomology andAgricultural Zoology, Benaki Phytopathological Institute, Athens, Greece), Danai Pervanidou (Directorate forEpidemiological Surveillance and Intervention for Infectious Diseases, Hellenic National Public Health Organization,Athens, Greece), Chantal Reusken (Centre for Infectious Disease Control, National Institute for Public Health andthe Environment, Bilthoven, the Netherlands), Flavia Riccardo (Department of Infectious Diseases, IstitutoSuperiore di Sanità, Rome, Italy), David Roiz (MIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, France), JavieraRebolledo Romero (Sciensano, Brussels, Belgium), Lucía García Sanmiguel (Coordinating Centre of Sanitary Alertsand Emergencies, Ministry of Health, Madrid, Spain), María José Sierra Moros (Coordinating Centre of SanitaryAlerts and Emergencies, Ministry of Health, Madrid, Spain), Luciano Toma (Department of Infectious Diseases,Istituto Superiore di Sanità, Rome, Italy), Henriette De Valk (Infectious Disease Division, Santé publique France,Paris, France), Paula Vasconcelos (Public Health Emergencies Operations Centre, Directorate-General of Health,Lisbon, Portugal), and Christiane Wagner-Wiening (Baden-Wuerttemberg State Health Office, Ministry of SocialAffairs, Health and Integration, Stuttgart, Germany.ECDC experts: Tamás Bakonyi, Olivier Briët, Céline Gossner, Joana Haussig, Nathalie Nicolay, Jenny MohseniSkoglund and Jonathan Suk.The authors acknowledge the valuable comments of Mohammed Afzal (ECDC), Sabrina Bacci (ECDC), Martina Brix-Zuleger (Ministry of Labour, Social Affairs, Health, Care and Consumer Protection, Vienna, Austria), Daniel Cauchi(ECDC), Áine Collins (ECDC), Sixtine Drougard (French National Agency for the Safety of Medicines and HealthProducts, Saint-Denis, France), Christina Frank (Department for Infectious Disease Epidemiology, Robert KochInstitute, Berlin, Germany), Helena de Carvalho Gomes (ECDC), Liese Van Gompel (ECDC), Phyo Myint (ECDC) andImad Sandid (French National Agency for the Safety of Medicines and Health Products, Saint-Denis, France).Suggested citation: European Centre for Disease Prevention and Control. Public health guidance for assessing andmitigating the risk of locally-acquiredAedes-borne viral diseases in the EU/EEA. Stockholm: ECDC; 2025.Stockholm, June 2025ISBN 978-92-9498-808-9doi: 10.2900/8091262Catalogue numberTQ-01-25-038-EN-N© European Centre for Disease Prevention and Control, 2025Reproduction is authorised, provided the source is acknowledged ContentsAbbreviations ............................................................................................................................................... ivExecutive summary ........................................................................................................................................1Background and scope ...................................................................................................................................3Key facts aboutAedes-borne viral diseases ......................................................................................................4Aedes-borne viruses.......................................................................................................................................4Epidemiology of dengue, chikungunya virus disease and Zika virus disease in Europe .....................................5Laboratory diagnosis ofAedes-borne virus infections ...................................................................................6Methodology of the guidance production..........................................................................................................8The risk matrix and suggested public health actions..........................................................................................9Definition of terms used to describe areas of transmission forAedes-borne virus infection...............................9Risk levels:...............................................................................................................................................9Knowledge gaps and limitations .................................................................................................