多实验室环试验，以支持经合组织关于Hyalella Azteca生物浓缩试验（HYBIT）的测试指南321的制定

Series on Testing and AssessmentNo. 398 Multi-Laboratory Ring Trial to SupportDevelopment of OECD Test Guideline 321 onHyalella Azteca Bioconcentration Test(HYBIT) 2024 Please cite this publication as: OECD (2024),Multi-Laboratory Ring Trial to Support Development of OECD Test Guideline 321 on Hyalella Azteca Bioconcentration Test(HYBIT), OECD Series on Testing and Assessment, No. 398, OECD Publishing, Paris. © OECD 2024 Attribution 4.0 International (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/). Attribution– you must cite the work. Translations– you must cite the original work, identify changes to the original and add the following text:In the event of any discrepancy between the original work and thetranslation, only the text of original work should be considered valid. Adaptations– you must cite the original work and add the following text:This is an adaptation of an original work by the OECD. The opinions expressed and argumentsemployed in this adaptation should not be reported as representing the official views of the OECD or of its Member countries. Third-party material– the licence does not apply to third-party material in the work. If using such material, you are responsible for obtaining permission from the third partyand for any claims of infringement. About the OECD The Organisation for Economic Co-operation and Development (OECD) is an intergovernmentalorganisation in which representatives of 38 countries in North and South America, Europe and the Asiaand Pacific region, as well as the European Union, meet to co-ordinate and harmonise policies, discussissues of mutual concern, and work together to respond to international problems. Most of the OECD’swork is carried out by more than 200 specialised committees and working groups composed of membercountrydelegates.Observers from several Partner countries and from interested internationalorganisations attend many of the OECD’s workshops and other meetings. Committees and workinggroups are served by the OECD Secretariat, located inParis, France, which is organised intodirectorates and divisions. The Environment, Health and Safety Division publishes free-of-charge documents in twelve differentseries:Testing and Assessment;Good Laboratory Practice and Compliance Monitoring;Pesticides;Biocides;Risk Management;Harmonisation of Regulatory Oversight inBiotechnology;Safety of Novel Foods and Feeds; Chemical Accidents; Pollutant Release andTransfer Registers; Emission Scenario Documents; Safety of Manufactured Nanomaterials;andAdverse Outcome Pathways.More information about the Environment, Health and Safety ProgrammeandEHSpublicationsisavailableontheOECD’sWorldWideWebsite(https://www.oecd.org/en/topics/chemical-safety-and-biosafety.html). This publication was developed in the IOMC context. The contents do not necessarily reflect theviews or stated policies of individual IOMC Participating Organizations. The Inter-Organisation Programme for the Sound Management of Chemicals (IOMC) wasestablishedin 1995 following recommendations made by the 1992 UN Conference onEnvironment and Development to strengthen co-operation and increase international co-ordination in the field of chemical safety. The Participating Organisations are FAO, ILO, UNDP,UNEP, UNIDO, UNITAR, WHO, World Bank, Basel, Rotterdam and Stockholm Conventions andOECD. The purpose of the IOMC is to promote co-ordination of the policies and activities pursuedby the Participating Organisations, jointly or separately, to achieve the sound management ofchemicals in relation to human health and the environment. Foreword This document presents the design and results of the ring trial on the Hyalella azteca bioconcentrationtest (HYBIT), in support of the development of Test Guideline 321 published in 2024. The ring trial reportwas endorsed by the Working Party of the National Coordinators of the Test Guidelines Programme(WNT) at their 36thmeeting in April 2024. This document is published under the responsibility of theChemicals and Biotechnology Committee. MULTI-LABORATORY RING TRIAL TO SUPPORT DEVELOPMENT OF OECD TESTGUIDELINE ONHYALELLA AZTECABIOCONCENTRATION TEST (HYBIT) TABLE OF CONTENTS TABLES................................................................................................................................................. 9FIGURES............................................................................................................................................... 9INTRODUCTION............................................................................................................................... 12MATERIALS AND METHODS........................................................................................................ 14Animal husbandry........................................................................................................................... 14Sexing of test animals............................................................