Act of 1934.Yes☐No☑Indicate by check mark whether the registrant (1)has filed all reports required to be filed by Section13 or 15(d)of the Securities Exchange Act of 1934 during the preceding 12months (or for such shorter period that the registrant was required to filesuch reports), and (2)has been subject to such filing requirements for the past 90days.Yes☑No☐Indicate by check mark whether the registrant has submitted electronically every Interactive Data File required to be submitted pursuant to Rule405 of RegulationS-T (§232.405 of this chapter) during the preceding 12months (or for such shorter period thatthe registrant was required to submit such files).Yes☑No☐ Table of Contents Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, a smallerreporting company, or an emerging growth company. See the definitions of “large accelerated filer,” “accelerated filer,” “smallerreporting company,” and “emerging growth company” in Rule12b‑2 of the Exchange Act. Accelerated filer☐Smaller reporting company☐Emerging growth company☐ Large accelerated filer☑ Non-accelerated filer☐ If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition periodfor complying with any new or revised financial accounting standards provided pursuant to Section 13(a) of the Exchange Act.☐Indicate by check mark whether the registrant has filed a report on and attestation to its management’s assessment of the effectiveness of its internal control over financial reporting under Section 404(b) of the Sarbanes-Oxley Act (15 U.S.C. 7262(b)) bythe registered public accounting firm that prepared or issued its audit report.☑If securities are registered pursuant to Section 12(b) of the Act, indicate by check mark whether the financial statements of the registrant included in the filing reflect the correction of an error to previously issued financial statements.☑Indicate by check mark whether any of those error corrections are restatements that required a recovery analysis of incentive- based compensation received by any of the registrant’s executive officers during the relevant recovery period pursuant to §240.10D-1(b).☑Indicate by check mark whether the registrant is a shell company (as defined in Exchange Act Rule12b‑2).Yes☐ No☑ The aggregate market value of the registrant’s Common Stock, $0.01par value, held by non-affiliates of the registrant as ofMarch 31, 2025, was approximately $1,003,383,280based on the closing price per share of $34.64 on March 31, 2025 on theNasdaq Stock Market. As of March 31, 2025, 45,776,018shares of the registrant’s Common Stock, $0.01par value, wereoutstanding. As ofDecember 1, 2025, 45,989,285shares of the registrant’s Common Stock, $0.01, par value, were outstanding. DOCUMENTS INCORPORATED BY REFERENCEPortions of the registrant’s Proxy Statement for the 2026Annual Meeting of Stockholders involving the election of directors, which is expected to be filed within 120days after the end of the registrant’s fiscalyear, are incorporated by reference in PartIII ofthis Annual Report on Form 10-K. AZENTA,INC. TABLE OF CONTENTS PARTI Item1.BusinessItem1A.Risk FactorsItem1B.Unresolved Staff CommentsItem 1C.CybersecurityItem2.PropertiesItem3.Legal ProceedingsItem4.Mine Safety DisclosuresPARTIIItem5.Market for Registrant’s Common Equity, Related Stockholder Matters and Issuer Purchases ofEquity SecuritiesItem6.[Reserved]Item7.Management’s Discussion and Analysis of Financial Condition and Results of OperationsItem7A.Quantitative and Qualitative Disclosures About Market RiskItem8.Financial Statements and Supplementary DataItem9.Changes in and Disagreements With Accountants on Accounting and Financial DisclosureItem9A.Controls and ProceduresItem9B.Other InformationItem 9C.Disclosure Regarding Foreign Jurisdictions that Prevent InspectionsPARTIIIItem10.Directors, Executive Officers and Corporate GovernanceItem11.Executive CompensationItem12.Security Ownership of Certain Beneficial Owners and Management and Related StockholderMattersItem13.Certain Relationships and Related Transactions, and Director IndependenceItem14.Principal Accountant Fees and ServicesPARTIVItem15.Exhibits and Financial Statement SchedulesItem16.Form 10-K SummarySIGNATURES INFORMATION RELATED TO FORWARD-LOOKING STATEMENTS This Annual Report on Form10-K containsforward-looking statements within the meaning of the Private Securities LitigationReform Act of 1995, as amended, Section 27A of the Securities Act of 1933, as amended, or the Securities Act, and Section 21E ofthe Securities Exchange Act of 1934, as amended, or the Exchange Act. Forward-looking statementsmay be identified by wordssuch as “expect,” “estimate,” “intend,” “believe,” “anticipate,” “may,” “will,” “should,” “could,” “continue,” “likely,” or similartermsor variations. Examplesof forward-looking statements include, but are not limited to, statements