Kartoon Studios, Inc. 4,784,909 Shares of Common Stock This Prospectus Supplement No. 1 supplements the prospectus dated January 19, 2024 (the “Prospectus”) relating to the resale fromtime to time of up to 4,784,909 shares (the “Shares”) of common stock, par value $0.001 per share (the “Common Stock”) of Kartoon The purpose of this ProspectusSupplementNo. 1 is solely to update the information in the table appearing under the caption “SellingStockholders” commencing on page 9 of the Prospectus to reflect in the Selling Stockholder table a transfer of a Warrant to purchaseup to 80,000 shares of Common Stock from Andrew Arno (“Arno”), a selling stockholder previously identified in the Prospectus, to *Represents less than 1% of the outstanding Common Stock.(1)Assumes the exercise in full of the Warrant held by the selling stockholder, without regard to any limitations on exercise (2)James Satloff is the Managing Member of Unterberg Legacy, LLC and, as a result, maintains voting and investment power with respect to the securities held by Unterberg Legacy, LLC. The address of Unterberg Legacy, LLC is 10 Gracie Square, New York, All of the other portions of the Prospectus remain unchanged. This Prospectus Supplement No. 1 is not complete without, and may not be utilized except in connection with, the Prospectus,including any amendments or supplements thereto. This Prospectus Supplement No. 1 is qualified by reference to the Prospectus,except to the extent that the information provided by this Prospectus Supplement No. 1 supersedes information contained in the Our Common Stock is listed on The NYSE American LLC (the “NYSE American”) under the symbol “TOON.” On June 9, 2026, thelast reported sale price of our Common Stock on the NYSE American was $0.75 per share. Investing in our securities involves risks. See “Risk Factors” beginning on page 7 of the Prospectus. Neither the Securities and Exchange Commission nor any state securities commission has approved or disapproved of thesesecurities or passed upon the adequacy or accuracy of the Prospectus or this Prospectus Supplement No. 1. Any representationto the contrary is a criminal offense. The date of this Prospectus Supplement No. 1 is June 10, 2026.