Form10-K (Mark One) ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year endedDecember 31,2024 TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Commission File Number:001-39555 GREENWICH LIFESCIENCES, INC.(Exact name of registrant as specified in its charter) (832)819-3232(Registrant’s telephone number, including area code)Securities registered pursuant to Section 12(b) of the Act: GLSI TheNASDAQCapital Market LLC Securities registered pursuant to Section 12(g) of the Act:None Indicate by check mark if the registrant is a well-known seasoned issuer, as defined in Rule 405 of the Securities Act. Yes☐No☒ Indicate by check if the registrant is not required to file reports pursuant to Section 13 or Section 15(d) of the Act. Yes☐No☒ Indicate by check mark whether the registrant (1) has filed all reports required to be filed by Section 13 or 15(d) of the SecuritiesExchange Act of 1934 during the preceding 12 months (or for such shorter period that the registrant was required to file such reports),and (2) has been subject to such filing requirements for the past 90 days.Yes☒No☐ Indicate by check mark whether the registrant has submitted electronically and posted on its corporate Web site, if any, everyInteractive Data File required to be submitted and posted pursuant to Rule 405 of Regulation S-T (§ 232.405 of this chapter) during thepreceding 12 months (or for such shorter period that the registrant was required to submit and post such files).Yes☒No☐ Indicate by check mark if disclosure of delinquent filers pursuant to Item 405 of Regulation S-K (§229.405 of this chapter) is notcontained herein, and will not be contained, to the best of registrant’s knowledge, in definitive proxy or information statementsincorporated by reference in Part III of this Form 10-K or any amendment to this Form 10-K.☒ Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, or a smallerreporting company. See the definitions of “large accelerated filer”, “accelerated filer”, “smaller reporting company”, and “emerginggrowth company” in Rule 12b-2 of the Exchange Act. Large accelerated filer☐Accelerated filer☐Non-accelerated filer☒Smaller reporting company☒Emerging growthcompany☒ If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period forcomplying with any new or revised financial accounting standards provided pursuant to Section 13(a) of the Exchange Act.☐ Indicate by check mark whether the registrant has filed a report on and attestation to its management’s assessment of theeffectiveness of its internal control over financial reporting under Section 404(b) of the Sarbanes-Oxley Act (15 U.S.C. 7262(b)) by theregistered public accounting firm that prepared or issued its audit report.☐ If securities are registered pursuant to Section 12(b) of the Act, indicate by check mark whether the financial statements of theregistrant included in the filing reflect the correction of an error to previously issued financial statements.☐ Indicate by check mark whether any of those error corrections are restatements that required a recovery analysis of incentive-based compensation received by any of the registrant’s executive officers during the relevant recovery period pursuant to §240.10D-1(b).☐ Indicate by check mark whether the registrant is a shell company (as defined in Rule 12b-2 of the Act). Yes☐No☒ The aggregate market value of the voting and non-voting common equity held by non-affiliates based on a closing sale price of $17.26per share, which was the last sale price of the common stock as of June 28, 2024, the last business day of the registrant’s most recentlycompleted second fiscal quarter, was approximately $101million. As of April 11, 2025,13,273,539 shares of the registrant’s common stock, $0.001 par value per share, were issued andoutstanding. TABLE OF CONTENTS PagePART IItem 1Business6Item 1ARisk Factors23Item 1BUnresolved Staff Comments54Item 1CCybersecurity54Item 2Properties54Item 3Legal Proceedings54Item 4Mine Safety Disclosures54 PART IIItem 5Market for Registrant’s Common Equity, Related Stockholder Matters and Issuer Purchases of Equity Securities55Item 6[Reserved]55Item 7Management’s Discussion and Analysis of Financial Condition and Results of Operations55Item 7AQuantitative and Qualitative Disclosures About Market Risk58Item 8Financial Statements and Supplementary Data58Item 9Changes In and Disagreements with Accountants on Accounting and Financial Disclosure58Item 9AControls and Procedures58Item 9BOther Information58Item 9CDisclosure Regarding Foreign Jurisdictions that Prevent Inspections.58PART IIIItem 10Directors, Executive Officers, and Corporate Governance59Item 11Executive Compensation63Item 12Security Ownership of Certain Beneficial Owners and Management and Related Stoc