“In just a few years, Prague has emerged as a strongcontender among the leading technology ecosystems ofCentral and Eastern Europe. Our tech economy is now valued at over €19 billion, representing more than 80% of theenterprise value of the entire Czech startup landscape. With 5 unicorns and hundreds ofambitious startups in the pipeline, Prague has become a true engine of innovation,resilience, and talent. The report also highlights what makes our ecosystem unique: the strength of ouruniversities, the creativity of our entrepreneurs, and the ability of local investors tomobilize unprecedented levels of funding. Looking ahead, Prague faces both opportunities and challenges. We must continue tonurture our thriving sectors — enterprise software, fintech, AI, deep tech, and lifesciences — while ensuring that growth translates into broader societal impact. We mustalso remain focused on talent development - the true gold here, educationmodernization, and openness to global partnerships, which will be critical inmaintaining our momentum. Tomáš LapáčekCEO The Prague Innovation Institute is proud to play its part in this journey — connectingvision with execution and ideas with impact - to foster Prague as not only a regionalleader, but a true European benchmark for innovation, creativity, and sustainability.” Page/ 2Page/ 2 1The Rise Of Prague Tech 2Ecosystem Today 3Outlook Pragueʼs techecosystem isnow worth€19.2B JetBrains, with a valuation of€6.4B, accounts for more than95% of the enterprise value ofnot VC Backed startups. 83% of Czechiaʼsenterprise valueis concentratedin Prague In CEE, Prague isthe third-largesthub after Kyivand Tallinn Prague also ranks third with8x growth since 2019.* Prague isshowing stronggrowth amongits Europeanpeer hubs Prague startup funnel, from rising stars to unicorns Among CEE hubs,Prague ranksfourth bynumber ofunicorns created When measured by unicornsper €1B VC invested and pernumber of funded startups,Prague falls behind peerhubs. By number ofthoroughbreds*, Pragueranks second in CEE afterKrakow. Prague shows astrongconversion fromVC funding to thenumber ofunicorns andthoroughbredscompared to theselectedEuropean hubs Prague has a pipeline of 300+ VC backed companies, including 8 unicorns andthoroughbreds Prague benchmarking against selected European ecosystems Universities playan importantrole in Pragueʼstech success,transformingresearch andeducation intoglobal startupleadership 1The Rise Of Prague Tech 2Ecosystem Today 3Outlook Praguecompanies areexpected to raise€165M of VC in2025 €90M was raised in H1 2025,representing 31% of theamount raised in H1 2024 and90% of the amount raised inH1 2023. Megarounds are still rare andhave been raised since 2019by a few companies, such asRohlik, Mews, Ataccama, andProductboard. » View online In 2025, theamount raised bybreakout-stagestartups isexpected toexceed €130M Mews with a €68.2M Late VCround and E2B with a €19.1MSeries A are the largestbreakout-stage rounds in 2025so far. Since 2019,Prague startupshave raised €2.5Bin VC funding, thesecond highestresult among CEEhubs Prague startups have a strong focus on Enterprise Software and Fintech Pipeline of Prague companies by stage and industry EnterpriseSoftware, Food,and Fintech havebeen the topindustries inPrague by VCraised since 2019 Explore the Prague startup ecosystem by industry Focus on Prague ecosystem industries Enterprise Software Energy €180MStartupscombined value €549MVC funding raisedsince 2019 €64MVC funding raisedsince 2019 €10.9BStartupscombined value €748MVC funding raisedsince 2019 €2.9BStartupscombined value 170+Startups 40+Startups 130+Startups Selected startups Selected startups Selected startups » Explore Prague Enterprise Softwarecompanies » Explore Prague Fintech companies » Explore Prague Energy companies Focus on Prague ecosystem industries €693MVC funding raisedsince 2019 €52MVC funding raisedsince 2019 €215MStartupscombined value €21MVC funding raisedsince 2019 €1.3BStartupscombined value €1BStartupscombined value 40+Startups 80+Startups Focus on Prague ecosystem industries Transportation €87MVC funding raisedsince 2019 €1.4BStartupscombined value €330MStartupscombined value €380MVC funding raisedsince 2019 €48MVC funding raisedsince 2019 €2.1BStartupscombined value 70+Startups 40+Startups 50+Startups Selected startups Selected startups Selected startups » Explore Prague transportationcompanies » Explore Prague Travel companies Looking at key segments such as AI, and Deep Tech & Life Sciences, Prague hasbeen among the top CEE hubs for VC investment since 2019 FOCUS ON AI AI sector is driven by a small number of startups, which account for almost ¼ ofthe combined enterprise value and ⅓ of the total VC investment raised since 2019 Defining AI In this context, we're exclusively examining"AI-first" companies—those where AI is central to their offerings. These companies typically fall int