Indicate by check mark whether the registrant (1)has filed all reports required to be filed by Section13 or 15(d)of the Securities Exchange Act of 1934 during the preceding 12months (or for suchshorter period that the registrant was required to file such reports), and (2)has been subject to such filing requirements for the past 90days.Yes☒No□ Indicate by check mark whether the registrant has submitted electronically every Interactive Data File required to be submittedpursuant to Rule405 of Regulation S-T (§ 232.405 of this chapter) duringthe preceding 12months (or for such shorter period that the registrant was required to submit such files).Yes☒No□ Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, a smaller reporting company, or an emerging growth company. See the definitions of“large accelerated filer,” “accelerated filer,” “smaller reporting company,” and “emerging growth company” in Rule12b-2 of the Exchange Act. Largeacceleratedfiler☐Acceleratedfiler☒Non-accelerated filer☐Smallerreportingcompany□Emerging growth company□ If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standardsprovided pursuant to Section13(a)of the Exchange Act.☐ Indicate by check mark whether the registrant has filed a report on and attestation to its management’s assessment of the effectiveness of its internal control over financial reporting under Section 404(b)of the Sarbanes-Oxley Act (15 U.S.C. 7262(b)) by the registered public accounting firm that prepared or issued its audit report.☒ If securities are registered pursuant to Section 12(b) of the Act, indicate by check mark whether the financial statements of the registrant included in the filing reflect the correction of an error topreviously issued financial statements.☐ Indicate by check mark whether any of those error corrections are restatements that required a recovery analysis of incentive-based compensation received by any of the registrant’s executive officersduring the relevant recovery period pursuant to § 240.10D-1(b).☐ Indicate by check mark whether the registrant is a shell company (as defined in Rule12b-2 of the Exchange Act).Yes☐No☒ The aggregate market value of common stock held by non-affiliates of the registrant as of June30, 2024 was $0.3billion based on the closing sale price of such common equity on such date (excluding1,061,389 shares of common stock held by directors and officers, and any stockholders whose ownership exceeds tenpercent of the shares outstanding as of June30, 2024). As of February28, 2025, there were49,910,391shares of the registrant’s common stock outstanding. DOCUMENTS INCORPORATED BY REFERENCE Portions of the registrant’s proxy statement for the 2024 Annual Meeting of Stockholders are incorporated herein by reference in Part III of this Annual Report on Form 10-K where indicated. Suchproxy statement will be filed with the U.S. Securities and Exchange Commission within 120 days of the registrant’s fiscal year ended December 31, 2024. TABLE OFCONTENTS PART I Item1.Business4Item1A.Risk Factors22Item1B.Unresolved Staff Comments35Item1C.Cybersecurity38Item2.Properties36Item3.Legal Proceedings36Item4.Mine Safety Disclosures36PARTIIItem5.Market for Registrant’s Common Equity, Related Stockholder Matters and Issuer Purchases of EquitySecurities37Item6.[Reserved]38Item7.Management’s Discussion and Analysis of Financial Condition and Results of Operations38Item7A.Quantitative and Qualitative Disclosures About Market Risk48Item8.Financial Statements and Supplementary Data49Item9.Changes in and Disagreements with Accountants on Accounting and Financial Disclosure49Item9A.Controls and Procedures49Item9B.Other Information50Item 9C.Disclosure Regarding Foreign Jurisdictions that Prevent Inspections50PARTIIIItem10.Directors, Executive Officers and Corporate Governance52Item11.Executive Compensation52Item12.Security Ownership of Certain Beneficial Owners and Management and Related Stockholder Matters52Item13.Certain Relationships and Related Transactions, and Director Independence52Item14.Principal Accountant Fees and Services52PARTIVItem15.Exhibits and Financial Statement Schedules53Item16.Form 10-K Summary56Signatures57 FORWARD-LOOKING STATEMENTS References to the “Company,” “Cryoport,” “we,” “us,” “our” and other similar words refer to Cryoport Inc.and its consolidated subsidiaries, unless the context suggests otherwise. This Annual Report on Form 10-K (this “Form10-K”) contains certain forward-looking statements. These forward-looking statements involve a number of risks anduncertainties. These forward-looking statements can generally be identified as such because the context of the statementwill include certain words, including but not limited to, “believes,” “may,” “will,” “expects,” “intends,” “estimates,”“anticipates,” “pla